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RESOURCES AND INSTRUMENTATION >
INSTRUMENTATION > MICROARRAYS
Microarrays
The Bauer Core has a number of instruments for creating, processing
and analyzing microarrays. For more information about designing an
experiment using either home-made or commercially available arrays please
see Microarray
Techniques & Protocols.
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Affymetrix GeneChip Instrumentation
The hybridization oven can accommodate 64 arrays and the two fluidics stations can each process up to four arrays at a time. The model 3000 7G scanner has a solid-state green laser which scans at 0.51 µm pixelation with reduced photobleaching. For more information, see http://www.affymetrix.com.
To learn about using this instrument, please contact
Jennifer Couget (jcouget@cgr.harvard.edu,
617-495-9402). |
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Agilent Scanner
This laser-based scanner can be used for Agilent microarrays and
homemade two-color (Cy3 and Cy5) microarrays. The carousel enables
automated scanning of up to 48 slides. For more information, see http://www.chem.agilent.com.
To learn about using this instrument, please contact
Claire Reardon (claire@cgr.harvard.edu,
617-384-7913).
Agilent
Scanner Protocol (MS Word) |
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Axon GenePix 4000B Scanners
These confocal laser scanners are used to analyze standard two-color
(Cy3 and Cy5) microarrays. GenePix software allows for adjustable
scan area and resolution, as well as automated spot finding. The
Genepix 4000B scans at resolutions between 5 and 100 microns. For
more information, see http://www.moleculardevices.com.
To learn about using this instrument, please contact
Christian Daly (cdaly@cgr.harvard.edu,
617-496-7105).
Axon GenePix
Protocol (MS Word) |
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Genomic Solutions OmniGrid
100
This high-throughput microarrayer has a capacity of 100 slides printed with
up to 48 pins. For more information, see http://www.genomicsolutions.com.
To
learn about using this instrument, please contact
Christian Daly (cdaly@cgr.harvard.edu,
617-496-7105).
OmniGrid
Protocol (MS Word) |
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