Nucleic Acid Shearing

The Covaris S220 system's controlled, reproducible DNA shearing method generates fragments with tight size distributions centered from 100 to 5000 bp. For more information see http://www.covarisinc.com/s_series.htm.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

Covaris Protocol (MS Word)

The Hydroshear is used for fragmenting DNA to sizes larger than 1KB. For more information see http://www.genomicsolutions.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

Hydroshear Protocol (MS Word)

Fisher 550 Sonicator (Misonix XL2020)

Used for DNA fragmentation and cell disruption. For more information see http://www.misonix.com.

To learn about using this instrument, please contact Claire Reardon (claire@cgr.harvard.edu, 617-384-7913).

Sonicator Protocol (MS Word)